All in one bag including injection port, 3lbs of sterilized corn and 3lbs of sterilized substrate (masters mix hardwood sawdust).

Colonize in the bag, fruit in the bag! Inject with your genetic material, then once the bottom layer of grain is colonize, mix into the top layer of substrate, allow to colonize, then introduce to fresh air, either by top fruiting or by slicing fruiting ports on the sides.